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KMID : 0191120230380320255
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Journal of Korean Medical Science
2023 Volume.38 No. 32 p.255 ~ p.255
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Sample Collection Methods in Upper Gastrointestinal Research
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Yang Hyo-Joon
Seo Seung-In
Lee Jin
Huh Cheal-Wung
Kim Joon-Sung
Park Jun-Chul
Kim Hyun-Ki
Shin Hak-Dong
Shin Cheol-Min
Park Chan-Hyuk
Lee Sang-Kil
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Abstract
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In recent years, significant translational research advances have been made in the upper gastrointestinal (GI) research field. Endoscopic evaluation is a reasonable option for acquiring upper GI tissue for research purposes because it has minimal risk and can be applied to unresectable gastric cancer. The optimal number of biopsy samples and sample storage is crucial and might influence results. Furthermore, the methods for sample acquisition can be applied differently according to the research purpose; however, there have been few reports on methods for sample collection from endoscopic biopsies. In this review, we suggested a protocol for collecting study samples for upper GI research, including microbiome, DNA, RNA, protein, single-cell RNA sequencing, and organoid culture, through a comprehensive literature review. For microbiome analysis, one or two pieces of biopsied material obtained using standard endoscopic forceps may be sufficient. Additionally, 5 mL of gastric fluid and 3?4 mL of saliva is recommended for microbiome analyses. At least one gastric biopsy tissue is necessary for most DNA or RNA analyses, while proteomics analysis may require at least 2?3 biopsy tissues. Single cell-RNA sequencing requires at least 3?5 tissues and additional 1?2 tissues, if possible. For successful organoid culture, multiple sampling is necessary to improve the quality of specimens.
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KEYWORD
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Endoscopy, Biopsy, Translational Research, Sample
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